Frequently asked questions about GenSolve
How long does the DNA recovery protocol take to perform? [open/close]
The protocol takes approximately 1 1/2 hours to perform.
Can the DNA recovery protocol be automated? [open/close]
Yes, we have worked with Hamilton liquid handler and can provide assistance in developing protocols for other liquid handlers to recovery DNA from 96 blood spotted elements at one time.
How much DNA can be recovered from each element and what is the total amount that can be recovered from the plate? [open/close]
The amount of DNA recovered varies based on the type of sample applied and the amount of genetic material present in that sample. Typically you can expect to obtain 50-300ng of DNA from a single element spotted with whole blood.
What concentration is the DNA upon recovery? [open/close]
The DNA is typically at a concentration of 0.8ng/ul.
What downstream applications are compatible with my eluted DNA? [open/close]
GenVault has specifically tested many downstream applications for compatibility: PCR, STR analysis, HLA typing and Whole Genome amplification are a few examples. Contact GenVault for data regarding your application.
How does the quality of GenVault recovered DNA compare to other commercial sources such as Qiagen preps? [open/close]
Performance in downstream applications such as PCR are equivalent. GenVault has amplified samples as long as 16kb from each procedure with no visible difference in quantity or quality.
How do I QC my DNA? [open/close]
You can use a PicoGreen assay for DNA quantitation, and a panel of PCR reactions to assess the quality of the DNA recovered from the GenPlate elements.