Stabilize purified RNA during sample handling, shipping and storage, with GenTegra™ RNA
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GenTegra™ RNA is an inert chemical matrix that inactivates trace RNase in the liquid phase, providing an added level of RNA stability during sample handling, with or without ice. In the dry-state, GenTegra™ preserves RNA integrity by protecting samples from hydrolysis and oxidation and imparts thermal stability at temperatures ranging from -80°C to 76°C /169°F, enabling ambient temperature storage and transport of RNA.
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Product Name |
Catalog # |
Description |
Units |
List Price |
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GTR 3112 |
Includes 10 GenTegra racks of 96 tubes each |
10 Racks |
1,850.00 |
GenTegra™ RNA Tubes Kit, Barcoded |
GTR3100 |
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1 kit |
$2,457.00 |
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GTR5025-S |
Pack of 25 GenTegra tubes |
25 tubes |
$51.00 |
GenTegra RNA Kit, Screw-Cap Tube, Starter |
GTR5001-S |
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1 kit |
$119.00 |
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GTR1501 |
10/pack |
$12.00 |
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Kit, Screw-Cap, 0.5mL |
GTR5201-S |
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25-Pack |
$13.00 |
To order from the catalog click click here or call 1.888.436.2005.
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Product |
GenTegra™ RNA |
Formats |
0.3 ml cluster tubes (rack of 96 tubes) |
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Application Volume |
20‐50μl |
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Application Amount |
1‐20μg |
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Concentration (RNA application) |
Any |
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Concentration (RNA recovery) |
Any |
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Drying Method |
FastDryer (overnight drying) |
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Increase the Stability of Purified RNA During Sample Handling, Shipping, or Long-Term Storage with GenTegra™
The integrity of RNA stored in GenTegra™ is equivalent to RNA stored frozen
Total RNA (20μg) purified from rat liver was stored in the dry state at 25°C for 30 days and compared with a control stored frozen at ‐80°C. RNA integrity was examined by running on a 0.8% agarose gel stained with ethidium bromide or using an Agilent Bioanalyzer.
Stabilization of PAXgene RNA in GenTegra™
The integrity of PAXgene RNA stored in the presence of GenTegra™ is quivalent to RNA stored frozen. RNA was purified from individual PAXgene tubes, and split into two aliquots. One aliquot of each sample was stored frozen at ‐80°C, while the other was stored in the dry state for 30 days at 25°C, 56°C or 76°C in the presence or absence of GenTegra™.
GenTegra Inactivates Trace RNase
RNA integrity is maintained in the liquid state in the presence of increasing amounts of RNase A and RNase 1 only when protected with GenTegra™. HeLa cell RNA (5μg) was incubated with the indicated amounts of RNase (unit is x109 molecule) at 37°C for one hour in the presence or absence of GenTegra™.
Illumina Expression Profiling with RNA recovered
from GenTegra™
Successful expression profiling of RNA using Illumina HT‐12 Expression Beadchips. Replicate RNA samples purified from HeLa cells (20μg) were stored in the dry state for two weeks at 25°C with GenTegra and compared with a control stored at ‐80°C.
Affymetrix Expression Profiling with RNA Recovered
from GenTegra™
Successful expression profiling of RNA using the Affymetrix GeneChip Human Genome U133 Plus 2.0 Array. Replicate RNA samples purified from HeLa cells (20μg) were stored in the dry state for two weeks at 25°C with GenTegra and compared with a control stored at ‐80°C.
MicroRNA Expression Profiling with RNA Recovered
from GenTegra™
Successful expression profiling of RNA using the Agilent miRNA microarray. Total RNA samples (20μg) containing miRNA were stored in the dry state for two weeks at 25°C with GenTegra and compared with a control stored at ‐80°C.
Click on the links below to download the reference materials:
Application Notes
Ambient Temperature Transport of purified RNA using GenTegra
Ambient Temperature Stablization of Cell line RNA for use in RT-qPCR Assays
Ambient Temperature Storage of RNA in GenTegra™ for use in a RT-qPCR test for the detection of GCC mRNA
Ambient Temperature Stabilization of Feline and Canine Tumor Cell RNA for Use in Gene Expression Assays
Stabilization of RNA for Laboratory Analysis, Shipment and Biobanking_POSTER.pdf
The Use of a Water-Soluble, Inert Chemical Matrix to Stabilize Purified RNA_poster.pdf
Stabilization of RNA in the liquid state at room temp-poster.pdf
Webinars
Ambient Temperature Stabilization of RNA derived from Feline and Canine Tumor Cells
Ambient Temperature Technology for Sustainable Biobanking of RNA for use in Expression Profiling